Alaa Salah, Hana AbdElaziz , Ragaa Satti



This study aims to investigate the methanol extract of Mustard (Brassica nigra L) seed, and to identify the chemical composition of the seed by chromatographic and spectroscopic methods based on the antimicrobial activity of the extract. Samples were taken from Khartoum local market, air dried, and cracked to be powder, in order to prepare the maceration methanol extract. The chemical properties of the Mustard (Brassica nigra L) Seed extracts were tested by phytochemical screening in our previous study which showed the availability of some components which were taken as an evidence for the presence of secondary metabolites. Results test of antimicrobial activity of methanol extract of seeds of Brassica nigra, proceeded by preliminary test using four bacteria (Escherichia coli, Staphylococcusaureus Bacillus subtilis, Pseudomonas eruginos). Our study showed that the methanolic extract (mac) of Brassica nigra have antimicrobial activity against all tested organisms. Thin Layer Chromatography (TLC) was applied using different solvents system. Intensive analytical investigations were carried out on the extract in order to identify and to isolate the effective components using column chromatography and preparative thin layer chromatography. The isolated pure fractions and analyzed by infra-red X-ray (IR) and ultra-violet (UV) spectroscopy. We recommend the benefit of medical plant such as Mustard (Brassica nigra L) Seed to explore possible future use of these extracts as alternative to common antibiotics and to determine their ability to enhance activities.

Keywords: Solvent extraction; Infra-red X-ray (IR); Column chromatography; Antibacterial activity