Syed Mubashar Sabir , Naveed Iqbal , Syed Rizwan Abbas and Nida Jabeen Abid DOWNLOAD PDF
ABSTRACT
The aim of the present study was to investigate the in vitro and in vivo antidiabetic effect of Elaeagnus umbellata, Phyllanthus emblica and Cannabis sativa. The in vitro antidiabetic activity was studied by the inhibition of alpha glucosidase enzyme. The in vivo antidiabetic activity was studied in albino mice. The diabetes was induced in mice by single interperitoneal injection of alloxan (150 mg/kg). The hot water extracts were fed to the mice orally (250 mg/kg) for seven days. The order of alpha glucosidase inhibitory effect was P. emblica > E. umbellata > C. sativa. The in vivo antidiabetic activity of extracts was further confirmed in alloxan induced diabetic mice. The Normal mice showed glucose level of 110±5 mg/dl. On treatment with single dose of alloxan (150 mg/kg) the sugar level was elevated to 250±10 mg/dl. The treatment with aqueous extract of E. umbellata fruit for seven days reduced the glucose level to 220±10 mg/dl. On the other hand treatment with aqueous extracts of P. emblica and C. sativa reduced the glucose level to 170±11 mg/dl and 185±12 mg/dl respectively. On the basis of these results it is concluded that studied plants have potential to decrease the glucose level by inhibiting the alpha glucosidase enzyme and potentiating of insulin release from pancreas. This will leads to the development of alternative drugs and therapeutic strategies.
KEYWORDS: E. umbellata, P. emblica, C. sativa, alpha glucosidase activity, alloxan, diabetes.